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Flow cytometry is a laser-based technique to count and analysis the size, shape and properties of individual cells within a heterogeneous population of cells. Flow cytometry is a widely used approach to phenotype the cells and to assessing the purity of isolated subpopulations. Predominantly, it is measured by the fluorescence intensity resulted from fluorescent-conjugated antibodies directly recognizing target proteins, or ligands specifically binding to molecules (such as DNA) within a cell. In addition, based on the properties of cells, a heterogeneous mixture of biological cells can be sorted into two or more containers by using a specialized type of flow cytometry called fluorescence-activated cell sorting (FACS).
About Flow Cytometry Guide:
Flow cytometry is a laser-based technique to count and analysis the size, shape and properties of individual cells within a heterogeneous population of cells. Flow cytometry is a widely used approach to phenotype the cells and to assessing the purity of isolated subpopulations. Predominantly, it is measured by the fluorescence intensity resulted from fluorescent-conjugated antibodies directly recognizing target proteins, or ligands specifically binding to molecules (such as DNA) within a cell. In addition, based on the properties of cells, a heterogeneous mixture of biological cells can be sorted into two or more containers by using a specialized type of flow cytometry called fluorescence-activated cell sorting (FACS).
Category: Biotechnology
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